Journal: Frontiers in Behavioral Neuroscience
Article Title: Contribution of CD4+ cells in the emotional alterations induced by endometriosis in mice
doi: 10.3389/fnbeh.2022.946975
Figure Lengend Snippet: Behavioral and neuroinflammatory alterations in endometriosis mice. (A) Timeline of the behavioral experiment and sample collection. Endometriosis mice showed (B) persistent mechanical hypersensitivity in the abdomino-pelvic area after stimulation with von Frey filaments, (C) increased anxiety-like behavior in the elevated plus maze test, (D) increased depressive-like behavior in the forced swimming test and (E) cognitive impairment in the novel object recognition task. [ (F) left panel] Endometriosis mice showed increased expression of interleukin 1-beta ( Il1b ), astrocyte marker GFAP ( Gfap ) and leucocyte marker CD4 ( Cd4 ) in periaqueductal gray; [ (F) central panel] overexpression of leucocyte markers CD4 ( Cd4 ) and CD2 ( Cd2 ) in medial prefrontal cortex, and [ (F) right panel] high levels of cyclooxygenase 2 ( Ptgs2 ), interleukin 1-beta ( Il1b ), CD4 ( Cd4 ) and CD2 ( Cd2 ) in hippocampus. Microglia marker IBA1 (Aif1) was unaltered. (G) Flow cytometry revealed 0.095 ± 0.042% GFP + cells in the peritoneum of WT mice 35 days after injecting GFP + endometrium. Error bars are mean ± SEM. (B) Mixed-model analysis (C–E) Student’s t -test. (F,G) Student’s t or Mann–Whitney U according to normality. ## p < 0.01, ### p < 0.001 vs baseline; * p < 0.05, ** p < 0.01, *** p < 0.001 vs control; + p < 0.05 vs wild-type. Endo, endometriosis; AUC, area under the curve; GFP, green fluorescent protein; WT, wild-type.
Article Snippet: Cells were then incubated 30 min on ice with DAPI (Sigma, cat.D9542), antiCD3 (1:100, CD3 Monoclonal (17A2), PE-Cyanine7, Invitrogen, cat.25-0032-82) and antiCD4 (1:100, FITC Rat AntiMouse CD4 Clone RM4-5, BD Biosciences, cat.553046).
Techniques: Expressing, Marker, Over Expression, Flow Cytometry, MANN-WHITNEY
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![Behavioral and neuroinflammatory alterations in endometriosis mice. (A) Timeline of the behavioral experiment and sample collection. Endometriosis mice showed (B) persistent mechanical hypersensitivity in the abdomino-pelvic area after stimulation with von Frey filaments, (C) increased anxiety-like behavior in the elevated plus maze test, (D) increased depressive-like behavior in the forced swimming test and (E) cognitive impairment in the novel object recognition task. [ (F) left panel] Endometriosis mice showed increased expression of interleukin 1-beta ( Il1b ), astrocyte marker GFAP ( Gfap ) and leucocyte marker <t>CD4</t> ( Cd4 ) in periaqueductal gray; [ (F) central panel] overexpression of leucocyte markers CD4 ( Cd4 ) and CD2 ( Cd2 ) in medial prefrontal cortex, and [ (F) right panel] high levels of cyclooxygenase 2 ( Ptgs2 ), interleukin 1-beta ( Il1b ), CD4 ( Cd4 ) and CD2 ( Cd2 ) in hippocampus. Microglia marker IBA1 (Aif1) was unaltered. (G) Flow cytometry revealed 0.095 ± 0.042% GFP + cells in the peritoneum of WT mice 35 days after injecting GFP + endometrium. Error bars are mean ± SEM. (B) Mixed-model analysis (C–E) Student’s t -test. (F,G) Student’s t or Mann–Whitney U according to normality. ## p < 0.01, ### p < 0.001 vs baseline; * p < 0.05, ** p < 0.01, *** p < 0.001 vs control; + p < 0.05 vs wild-type. Endo, endometriosis; AUC, area under the curve; GFP, green fluorescent protein; WT, wild-type.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6757/pmc09596757/pmc09596757__fnbeh-16-946975-g001.jpg)
